12/2/2023 0 Comments Principle of western blot test![]() ![]() The protein or polypeptide used as an antigen on the solid phase carrier triggers an immune response for the relevant antibody, and then reacts with secondary antibodies labeled by enzymes or isotopes. The solid phase carrier adsorbs proteins in the noncovalent form and can keep polypeptide type separated by electrophoresis and biological activity unchanged. Protein samples separated by PAGE are transferred to solid phase carrier (e.g. ![]() The labeled secondary antibody is used for staining. Similar to southern blot or northern blot hybridization technique, but western blot uses polyacrylamide gel electrophoresis (PAGE). Currently, western blot is widely used in expression of gene at protein level, antibody activity test and early diagnosis of diseases etc. Western blot is a kind of protein detection technique, which transfers cell or total protein separated by electrophoresis from gel to solid phase carrier like NC membrane or PVDF membrane and then test a specific antigen by specific antibody. This protein method was first known as western blot in the Analytical Biochemistry written by Neal Burnette in 1981. Western blot was proposed by Harry Towbin from Switzerland Friedrich Miescher Institute in 1979. Then, the information of specific protein expressed in analyzed cell or tissue is obtained. Cell or biological tissue samples processed by gel electropherosis are stained by specific antibodies. Western blot is a common experimental method in molecular biology, biochemistry and immunogenetics. Keywords: Western Blot Step by Step, Protein Analysis Methods, Molecular Biology Techniques, Western Blot Antibody, FineTest Antibody 1. Especially, there are great influences and development space in medical applications. Abstract: Western blot has been widely applied in modern biological research for qualitative and semi-quantitative protein analysis since the invention. ![]()
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